Cellulase From Aspergillus Niger 25G powder, off-white, ~0.8 U/mg General description Cellulase is a member of glycoside hydrolase family and is produced by a number of cellulolytic microbes. Application-Cellulase from Aspergillus niger has been used:
- in the preparation of the standardized solution to determine absolute cellulase levels. - in phosphate-citric acid buffer for activity assay - in non-starch polysaccharide degrading enzyme cocktail for in vitro hydrolysis of defatted rice bran and in the approach to improve the nutritive value of poultry feed. Biochem/physiol Actions - Cellulase from Aspergillus niger catalyzes the hydrolysis of endo-1,4-β-D-glycosidic linkages in cellulose, lichenin, barley glucan, and the cellooligosaccharides cellotriose to cellohexaose. It does not cleave cellobiose or p-nitrophenyl-β-D-glucoside. This enzyme will also cleave intact glycosaminoglycan from a core peptide by hydrolyzing the xylosyl serine linkage. Other Notes - 1 U corresponds to the amount of enzymes hich liberates 1 μmol glucose from carboxymethylcellulose per minute at pH 5.0 and 37°C
CAS Number: 9012-54-8
UN No: 232-734-4
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